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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC Ch Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB9354 Replaces 04-1049 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Beta III Tubulin Antibody 背景信息 Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue. 产品信息 格式 Affinity Purified 控制 - Brain tissue, Neuronal culture
演示 Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product应用 应用 Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.
Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.
Optimal working dilutions must be determined by the end user生物信息 免疫原品种 Synthetic peptides from human/rat Beta III Tubulin. 表位 (Similar to TuJ1 antigen) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 Recognizes Beta III Tubulin 同种型 IgY 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006086.2
基因符号 - MC1R
- TUBB3
- TUBB4
- beta-4
纯化方法 Immunoaffinity purified UniProt编号 - Q13509
UniProt汇总 FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.包装信息 数量 500 µL
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