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merck millipore,默克密理博,ABS150,Anti-phospho-PDGFRα (Tyr754) Antibody
产品名称：Anti-phospho-PDGFRα (Tyr754) Antibody
产品型号：ABS150
Detect phospho-PDGFRα (Tyr754) using this Anti-phospho-PDGFRα (Tyr754) Antibody validated for use in Western Blotting.
merck millipore,默克密理博,ABS150,Anti-phospho-PDGFRα (Tyr754) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 B, M, H, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS150 描述 Anti-phospho-PDGFRα (Tyr754) Antibody Alternate Names - CD_antigen=CD140a
- Platelet-derived growth factor receptor alpha
- PDGF-R-alpha
- PDGFR-alpha
- Alpha platelet-derived growth factor receptor
- Alpha-type platelet-derived growth factor receptor
- CD140 antigen-like family member A
- CD140a antigen
- Platelet-derived growth factor alpha receptor
- Platelet-derived growth factor receptor 2
- Short name=PDGFR-2
背景信息 PDGFRα (platelet-derived growth factor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A) is a ubiquitous receptor tyrosine kinase. It is activated by the binding of homo- or heterodimers of PDGFA, PDGFB or PDGFC. Upon ligand binding, PDGFRα dimerizes as two alpha or two beta subunits depending on the cellular context, and is autophosphorylated at multiple tyrosine residues. Phosphorylated tyrosine residues interact with different targets to mediate cell proliferation, migration, and survival. Phosphorylation at Tyrosine 754 induces heterodimerization and allows PDGFRα to interact with PTPN11. In addition, PDGRα regulates apoptosis by multiple signaling pathways that converge on proapoptotic proteins such as BAD. For instance, PDGFRα signaling deactivates BAD by induction of PI3K/Akt; PLC-gamma/PKC/Bcl-2; and Ras/Raf/Erk signaling pathways. Defective PDGFRα has been implicated in hypereosinophilic syndrome and gastrointestinal stromal tumor. 产品信息 格式 Affinity Purified 控制 - Untreated and PDGF treated NIH/3T3 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect phospho-PDGFRα (Tyr754) using this Anti-phospho-PDGFRα (Tyr754) Antibody validated for use in Western Blotting. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human PDGFRα phosphorylated at (Tyr754). 表位 Phosphorylated (Tyr754) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes PDGFRα phosphorylated at (Tyr754). 品种反应性 BovineMouseHumanRat Species Reactivity Note Demonstrated to react with Mouse. Predicted to react with Human, Rat, and Bovine based on 100% sequence homology. Other homologies: Xenopus and Chicken (91% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006197
Entrez基因汇总 This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq, Jul 2008]. 基因符号 - RHEPDGFRA
- PDGFRA
- PDGFR2
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - P16234
UniProt汇总 FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Present in an inactive conformation in the absence of bound ligand. Binding of PDGFA and/or PDGFB leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by imatinib, nilotinib and sorafenib.
SUBUNIT STRUCTURE: Interacts with homodimeric PDGFA, PDGFB and PDGFC, and with heterodimers formed by PDGFA and PDGFB. Monomer in the absence of bound ligand. Interaction with dimeric PDGFA, PDGFB and/or PDGFC leads to receptor dimerization, where both PDGFRA homodimers and heterodimers with PDGFRB are observed. Interacts (tyrosine phosphorylated) with SHB (via SH2 domain) (By similarity). Interacts (tyrosine phosphorylated) with SHF (via SH2 domain). Interacts (tyrosine phosphorylated) with SRC (via SH2 domain). Interacts (tyrosine phosphorylated) with PIK3R1. Interacts (tyrosine phosphorylated) with PLCG1 (via SH2 domain). Interacts (tyrosine phosphorylated) with CRK, GRB2 and GRB7. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note: The activated receptor is rapidly internalized and degraded.
TISSUE SPECIFICITY: Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
PTM: N-glycosylated.
Ubiquitinated, leading to its degradation Probable.
Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1.
INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del4(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). Mutations that cause overexpression and/or constitutive activation of PDGFRA may be a cause of hypereosinophilic syndrome.
Defects in PDGFRA are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. Note=Mutations that cause constitutive activation of PDGFRA may be a cause of gastrointestinal stromal tumor (GIST).
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
SEQUENCE CAUTION: The sequence AAP69563.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in untreated and PDGF treated NIH/3T3 cell lysates.
Western Blot Analysis: 2.5 µg/mL of this antibody detected PDGFRα on 10 µg of untreated and PDGF treated NIH/3T3 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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