merck millipore,默克密理博,ABS107,Anti-phospho-LRRK2 (Thr1503) Antibody

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merck millipore,默克密理博,ABS107,Anti-phospho-LRRK2 (Thr1503) Antibody
产品名称：Anti-phospho-LRRK2 (Thr1503) Antibody
产品型号：ABS107
Anti-phospho-LRRK2 (Thr1503) Antibody detects level of phospho-LRRK2 (Thr1503) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.

merck millipore,默克密理博,ABS107,Anti-phospho-LRRK2 (Thr1503) Antibody

产品介绍

merck millipore,默克密理博,ABS107,Anti-phospho-LRRK2 (Thr1503) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Po, H, Bab, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABS107
描述	Anti-phospho-LRRK2 (Thr1503) Antibody
Alternate Names	Dardarin Leucine-rich repeat serine/threonine-protein kinase 2
背景信息	Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.

产品信息
格式	Affinity Purified
控制	Cell lysates transfected with truncated forms of wild-type LRRK2 with auto-phosphorylation properties and mutant D1994A LRRK2 which lacks auto-phosphorylation properties.
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-phospho-LRRK2 (Thr1503) Antibody detects level of phospho-LRRK2 (Thr1503) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.
主要应用	Western Blotting
应用说明	Peptide Inhibition Analysis: 0.2 µg/mL from representative lot blocked phospho-LRRK2 (Thr1503) in cell lysates transfected with truncated wild-type LRRK2.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to the ROC domain of human LRRK2 phosphorylated at Thr1503.
表位	ROC domain
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes the ROC domain of LRRK2 phosphorylated at Tyr1503.
品种反应性	PigHumanBaboonMouseRat
Species Reactivity Note	Demonstrated to react with Human. Predicted to react with Baboon, Mouse, Rat, Porcine based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_940980
Entrez基因汇总	This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
基因符号	PARK8 LRRK2
修改	Phosphorylation
纯化方法	Affinity purified
UniProt编号	Q5S007
UniProt汇总	FUNCTION: Probable protein kinase whose role is not yet known. May play a role in the phosphorylation of proteins central to Parkinson disease. May also have GTPase activity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT STRUCTURE: Interacts with PARK2. SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane. TISSUE SPECIFICITY: Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. INVOLVMENT IN DISEASE: Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

产品使用声明
质量保证	Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity. Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Thr1503) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity.

Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Thr1503) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.