merck millipore,默克密理博,06-579,Anti-Gab1 Antibody, CT

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merck millipore,默克密理博,06-579,Anti-Gab1 Antibody, CT
产品名称：Anti-Gab1 Antibody, CT
产品型号：06-579
Anti-Gab1 Antibody, CT detects level of Gab1 & has been published & validated for use in IC, IH(P), IP & WB.

merck millipore,默克密理博,06-579,Anti-Gab1 Antibody, CT

产品介绍

merck millipore,默克密理博,06-579,Anti-Gab1 Antibody, CT

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-579
品牌系列	Upstate
商名	Upstate
描述	Anti-Gab1 Antibody, CT
Alternate Names	Growth factor receptor bound protein 2-associated protein GRB2-associated binder GRB2-associated binding protein
背景信息	Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFR-β]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

产品信息
格式	Purified
控制	NIH/3T3 cytoplasmic lysate. Included Positive Antigen Control: Catalog # 12-301 non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示	Purified IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

应用
应用	Anti-Gab1 Antibody, CT detects level of Gab1 & has been published & validated for use in IC, IH(P), IP & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: 4 μg of this lot immunoprecipitated Gab1 from 500 μg of a human A431 carcinoma cell RIPA lysate. Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Gab1 in A431 cells fixed with 4% paraformaldehyde.

生物信息
免疫原品种	31 residue peptide sequence corresponding to C-terminal residues 664-694 of Gab1, (CQKTLALKSTREAWTDGRQSTESETPAKSVK).
表位	C-terminus
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Specific for Gab1. No other known protein shares more than 10 amino acids with the immunizing sequence.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse and rat. Reactivity with other species has not been confirmed.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_207123
Entrez基因汇总	The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
基因符号	GAB1
纯化方法	Protein A purfied
UniProt编号	Q13480
UniProt汇总	FUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling. SIZE: 694 amino acids; 76616 Da SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on NIH/3T3 cytoplasmic lysate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Gab1 in RIPA lysates from human A431 carcinoma cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.