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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Gp Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN90P 描述 Anti-NeuN purified Antibody Alternate Names - NeuN
- NEUronal Nuclei
- clone A60
背景信息 ABN90P is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. 产品信息 格式 Affinity Purified 控制 - Mouse E16 brain tissue lysate
演示 Purified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-NeuN purified Antibody is validated for use in Western Blotting, ICC, IHC(P) for the detection of NeuN purified. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse frontal cortex tissue.
Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Guinea Pig 特异性 This antibody recognizes the N-terminus of NeuN. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Mouse, Rat, and Human. 抗体类型 Polyclonal Antibody 基因符号 - NeuN
纯化方法 Antigen Affinity purified 产品使用声明 质量保证 Evaluated by Western Blot in mouse E16 brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,46-651MAG,MILLIPLEX MAP Phospho c-Met/HGFR (panTyr) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay
下一件merck millipore产品：merck millipore,默克密理博,AP127P,Goat Anti-Mouse IgG Antibody, Fc, HRP conjugate
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