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品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, Ca, Rhesus Macaque, Eq, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN68 描述 Anti-Bystin Antibody 背景信息 Bystin and tastin serve as cytoplasmic proteins that are required for trophinin to mediate cell-to-cell adhesion between trophoblastic and endometrial epithelial cells in human placenta. Although the trophinin gene is unique to mammals, the bystin gene (BYSL) is conserved across eukaryotes. It is shown that bystin influences the transition from silent trophectoderm to an active trophoblast through trophinin-mediated cell adhesion. It is also essential for embryonic stem cell survival and trophectoderm development in mouse. Bystin is demonstrated to play a key role in ribosomal biogenesis by processing 18S RNA in the 40S subunit in human cancer cells and mouse embryos. It is also reported that c-MYC targets BYSL, which corresponds with bystin-mediated, rapid protein synthesis, and subsequent cellular growth. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Bystin Antibody is an antibody against Bystin for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Bystin in the syncytiotrophoblastic layer of the chorionic villi in human placenta. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of human Bystin. 表位 C-terminus. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminus of Bystin. 品种反应性 BovineHumanMouseCanineRhesus MacaqueHorseRat Species Reactivity Note Demonstrated to react with Human and Mouse. Predicted to react with Canine, Rhesus Macaque, Equine, Rat, and Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004044
Entrez基因汇总 Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. 基因符号 - BYSL
纯化方法 Affinity purified UniProt编号 - Q13895
UniProt汇总 FUNCTION: Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos.
SUBUNIT STRUCTURE: Binds trophinin, tastin and cytokeratins.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Associated with 40S ribosomal subunits.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Bystin in 10 µg HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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