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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN285 描述 Anti-ZEB1 Antibody Alternate Names - TCF-8
- Zinc finger E-box-binding homeobox 1
- NIL-2-A zinc finger protein
- Negative regulator of IL2
- Transcription factor 8
背景信息 ZEB1 (zinc-finger-enhancer binding protein 1) is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. ZEB1 is expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Mutations in ZEB1 have been associated with posterior polymorphous corneal dystrophy type 3 which is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. 产品信息 格式 Affinity Purified 控制 - HeLa nuclear extract
演示 Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide and 50% glycerol. 应用 应用 This ZEB1 antibody is validated for use in WB & ICC for the detection of the ZEB1 protein. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ZEB1 in HeLa cells. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human ZEB1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody 基因符号 - TCF8
- ZEB1
- AREB6
纯化方法 Antigen Affinity purified UniProt编号 - P37275
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZEB1 in 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 150 µL
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