merck millipore,默克密理博,ABF234,Anti-TFEB Antibody

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merck millipore,默克密理博,ABF234,Anti-TFEB Antibody
产品名称：Anti-TFEB Antibody
产品型号：ABF234
Detect TFEB using this rabbit polyclonal antibody, Anti-TFEB Antibody validated for use in western blotting, IHC & Immunofluorescence.

merck millipore,默克密理博,ABF234,Anti-TFEB Antibody

产品介绍

merck millipore,默克密理博,ABF234,Anti-TFEB Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABF234
描述	Anti-TFEB Antibody
Alternate Names	TFEB Transcription factor EB Class E basic helix-loop-helix protein 35 bHLHe35
背景信息	The protein named Transcription factor EB or alternatively in older literature Class E basic helix-loop-helix protein 35 (bHLHe35), and encoded by the human gene TFEB/BHLH35, is a transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') on DNA. Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. TFEB in association with the protein TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also plays a central role in expression of lysosomal genes. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes and through binding acts as a positive regulator of their expression. Finally, TFEB appears to be a positive regulator for autophagy related gene expression, heavy chain immunoglobulin gene expression and genes necessary for the proper vascularization of important tissues such as the placenta. EMD-Millipore’s Anti-TFEB rabbit polyclonal antibody has been tested in western blotting on Jurkat cell extracts, paraffin embedded immunohistochemistry on human renal cell carcinoma tissue sections and immunofluorescence on HeLa cells.

产品信息
格式	Affinity Purified
演示	Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.

应用
应用	Detect TFEB using this rabbit polyclonal antibody, Anti-TFEB Antibody validated for use in western blotting, IHC & Immunofluorescence.
主要应用	Immunohistochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TFEB in human renal cell carcinoma tissue. Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected TFEB in HeLa cells.

生物信息
免疫原品种	GST-tagged fusion protein corresponding to human TFEB.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	Human
抗体类型	Polyclonal Antibody
基因符号	BHLHE35 TFEB
纯化方法	Antigen Affinity purified
UniProt编号	P19484

产品使用声明
质量保证	Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFEB in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.