merck millipore,默克密理博,ABE621,Anti-EIF4A2 Antibody

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merck millipore,默克密理博,ABE621,Anti-EIF4A2 Antibody
产品名称：Anti-EIF4A2 Antibody
产品型号：ABE621
Detect EIF4A2 using this rabbit polyclonal antibody, Anti-EIF4A2 Antibody validated for use in western blotting & IHC.

merck millipore,默克密理博,ABE621,Anti-EIF4A2 Antibody

产品介绍

merck millipore,默克密理博,ABE621,Anti-EIF4A2 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABE621
描述	Anti-EIF4A2 Antibody
Alternate Names	EIF4A2 Eukaryotic initiation factor 4A-II eIF-4A-II eIF4A-II ATP-dependent RNA helicase eIF4A-2
背景信息	Eukaryotic initiation factor 4A-II, (EIF4A2), or ATP-dependent RNA helicase eIF4A-2 is the critical RNA helicase protein required for mRNA cap recognition and the mRNA binding to the ribosome. EIF4A2 is part of a multi-subunit complex that allows translation of mRNAs to begin. EIF4A2 unwinds the RNA secondary structure in the 5’-UTR region and thus allows the small ribosomal subunit to bind the mRNA and begin scanning for the initiator codon. EIF4A2 also plays a key role in mRNA expression inhibition; research demonstrates that inhibition of EIF4A2 function via microRNA inhibition is required first, which then leads to mRNA destabilization and ultimately translation suppression. EIF4A2 is expressed in all tissues as well as in cancerous tumors with skeletal muscle and ovary being very high, followed by liver, kidney, brain and pancreas in lower amounts. EIF4A2 is often used as a “housekeeping” marker as well because of its wide expression. EMD-Millipore’s Anti-EIF4A2 rabbit polyclonal antibody has been tested in western blots on Jurkat cell lysates and in paraffin embedded immunohistochemistry on human ovarian tumor tissue sections.

产品信息
格式	Affinity Purified
演示	Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.

应用
应用	Detect EIF4A2 using this rabbit polyclonal antibody, Anti-EIF4A2 Antibody validated for use in western blotting & IHC.
主要应用	Immunohistochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected EIF4A2 in human ovary tumor tissue.

生物信息
免疫原品种	GST-tagged fusion protein corresponding to human EIF4A2.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	Human
抗体类型	Polyclonal Antibody
基因符号	EIF4F EIF4A2 DDX2B
纯化方法	Antigen Affinity purified
UniProt编号	Q14240

产品使用声明
质量保证	Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: A 1:400 dilution of this antibody detected EIF4A2 in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.