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merck millipore,默克密理博,ABE365,Anti-LSD1 Antibody
产品名称：Anti-LSD1 Antibody
产品型号：ABE365
Use Anti-LSD1 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect LSD1 also known as Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110.
merck millipore,默克密理博,ABE365,Anti-LSD1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE365 Replaces MABE545 描述 Anti-LSD1 Antibody Alternate Names - Flavin-containing amine oxidase domain-containing protein 2
- Lysine-specific histone demethylase 1A
- BRAF35-HDAC complex protein BHC110
背景信息 LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. 产品信息 格式 Affinity Purified 控制 - HeLa nuclear extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-LSD1 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect LSD1 also known as Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated LSD1 in 0.5 mg of HeLa nuclear extract. 生物信息 免疫原品种 Histidine-tagged recombinant protein corresponding to full length LSD1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes LSD1. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_055828
Entrez基因汇总 This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. 基因符号 - LSD1
- KDM1A
- AOF2
- KDM1
纯化方法 Affinity purified UniProt编号 - O60341
UniProt汇总 FUNCTION: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
COFACTOR: FAD.
SUBUNIT STRUCTURE: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitously expressed.产品使用声明 质量保证 Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 in 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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