- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,05-1313,Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
产品名称：Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
产品型号：05-1313
Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
merck millipore,默克密理博,05-1313,Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt WB, ELISA, IHC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-1313 描述 Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 Alternate Names - ubiquitin B
- ubiquitin C
- polyubiquitin B
背景信息 Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis. 产品信息 格式 Purified 控制 - Lys63 polyubiquitin chains.
演示 Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide. 应用 应用 Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
- Immunocytochemistry
应用说明 Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications
Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.
Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer's patient.
ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.
生物信息 免疫原品种 Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST). 表位 Lys63 ubiquitination site 克隆 HWA4C4 宿主 Mouse 特异性 Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin. 同种型 IgG2a 品种反应性 Vertebrates Species Reactivity Note Human. Based on sequence homology, broad species cross-reactivity is expected. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_018955.2
Entrez基因汇总 This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]
基因符号 - FLJ25987
- MGC8385
- OTTHUMP00000064960
- OTTHUMP00000064961
- UBA80
- UBCEP1
- UBCEP2
- UBB
- UBC
UniProt编号 - P62988
UniProt汇总 FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIMILARITY: Belongs to the ubiquitin family.
产品使用声明 质量保证 Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,SF-1529,CCR5; Human, Cyanine 3 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,07-234,Anti-REA Antibody
相关产品
merck millipore,默克密理博,374805,Hemocyanin, Keyhole Limpet, Megathura crenulata
merck millipore,默克密理博,ABE365,Anti-LSD1 Antibody
merck millipore,默克密理博,5710-OP,OmniPur Imidazole - CAS 288-32-4 - Calbiochem
merck millipore,默克密理博,103256,卡可酸钠盐
merck millipore,默克密理博,SF-1529,CCR5; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,07-234,Anti-REA Antibody
merck millipore,默克密理博,LIF1050,Leukemia Inhibitory Factor, recombinant human
merck millipore,默克密理博,07-049,Anti-Nedd4 Antibody, WW2 domain
merck millipore,默克密理博,MABN666,Anti-TRIP8b Antibody, clone N212A/34
