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  • merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

  • merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

    产品名称：Anti-trimethyl Histone H3 (Lys36) Antibody
    产品型号：ABE305
    Use Anti-trimethyl Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP to detect trimethyl Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).

    merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

  • 产品介绍
  • merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HChIP, WB, ICCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 ABE305
    描述 Anti-trimethyl Histone H3 (Lys36) Antibody
    Alternate Names
    • H3k36me3
    • Histone H3.1
    • Histone H3/a
    • Histone H3/b
    • Histone H3/c
    • Histone H3/d
    • Histone H3/f
    • Histone H3/h
    • Histone H3/I
    • Histone H3/j
    • Histone H3/k
    • Histone H3/l
    背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.
    产品信息
    格式 Affinity Purified
    控制
    • Histone H3 recombinant proteins
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Use Anti-trimethyl Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP to detect trimethyl Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    • Immunocytochemistry
    应用说明 Dot Blot Analysis: A 1:500 dilution from a representative lot detected trimethyl Histone H3 (Lys36).

    Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in mouse embryonic stem cells. (Laboratory of Dr. Rob Klose, Oxford University)

    Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NIH/3T3 fibroblasts. (Laboratory of Dr. Rob Klose, Oxford University)
    生物信息
    免疫原品种 Linear peptide corresponding to human Histone H3 trimethylated at Lys36.
    表位 Trimethylated Lys36
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes Histone H3 trimethylated at Lys36.
    品种反应性 MouseHuman
    Species Reactivity Note Demonstrated to react with Human and Mouse.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_003520
    Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].
    基因符号
    • H3FJ
    • HIST1H3A
    • H3FA
    • HIST1H3B
    • H3FL
    • HIST1H3C
    • H3FC
    • HIST1H3D
    • H3FB
    • HIST1H3E
    • H3FD
    • HIST1H3F
    • H3FI
    • HIST1H3G
    • H3FH
    • HIST1H3H
    • H3FK
    • HIST1H3I
    • H3FF
    • HIST1H3J
    修改
    • Methylation
    纯化方法 Affinity purified
    UniProt编号
    • P68431
    UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

    SUBCELLULAR LOCATION: Nucleus. Chromosome.

    DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

    PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).

    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.

    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.

    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.

    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.

    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.

    MISCELLANEOUS: This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).

    SEQUENCE SIMILARITIES: Belongs to the histone H3 family.
    产品使用声明
    质量保证 Evaluated by Western Blot in Histone H3 recombinant proteins.

    Western Blot Analysis: A 1:2,000 dilution of this antibody detected Histone H3 on 10 µg of Histone H3 recombinant proteins.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

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