merck millipore,默克密理愽
  • merck millipore,默克密理博,16-105,Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate

  • merck millipore,默克密理博,16-105,Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate

    产品名称：Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate
    产品型号：16-105
    Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in ELISA & WB.

    merck millipore,默克密理博,16-105,Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate

  • 产品介绍
  • merck millipore,默克密理博,16-105,Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    AWB, ELISAMHRPMonoclonal Antibody
    描述
    产品目录编号 16-105
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate
    概述 Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
    背景信息 Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.

    Description
    IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
    产品信息
    格式 HRP
    控制
    • Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
    演示 200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% ProClin®150. Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
    应用
    应用 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in ELISA & WB.
    主要应用
    • Western Blotting
    • ELISA
    应用说明 For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
    生物信息
    免疫原品种 Phosphotyramine-KLH.
    表位 N/A
    克隆 4G10®
    宿主 Mouse
    特异性 Recognizes tyrosine-phosphorylated proteins from all species.
    同种型 IgG2bκ
    品种反应性 All
    Species Reactivity Note All
    抗体类型 Monoclonal Antibody
    修改
    • Phosphorylation
    纯化方法 Protein G-Sepharose™ chromatography
    产品使用声明
    质量保证 Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.

    Western Blot Analysis:
    Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
    NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
    包装信息
    数量 10 blots

    merck millipore,默克密理博,16-105,Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate

上一件merck millipore产品：merck millipore,默克密理博,516854,Pertussis Toxin, A Protomer, Bordetella pertussis - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,MAB1962,Anti-Integrin β2 Antibody, clone P4H9


相关产品

merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody merck millipore,默克密理博,ABE305,Anti-trimethyl Histone H3 (Lys36) Antibody

merck millipore,默克密理博,SF-1060,PDX1; Human, Cyanine 3 RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-1060,PDX1; Human, Cyanine 3 RNA Detection Probe,SmartFlare

merck millipore,默克密理博,178464,Anti-Apolipoprotein A-II Goat pAb merck millipore,默克密理博,178464,Anti-Apolipoprotein A-II Goat pAb

merck millipore,默克密理博,ABT330,Anti-TSLP Antibody merck millipore,默克密理博,ABT330,Anti-TSLP Antibody

merck millipore,默克密理博,516854,Pertussis Toxin, A Protomer, Bordetella pertussis - Calbiochem merck millipore,默克密理博,516854,Pertussis Toxin, A Protomer, Bordetella pertussis - Calbiochem

merck millipore,默克密理博,MAB1962,Anti-Integrin β2 Antibody, clone P4H9 merck millipore,默克密理博,MAB1962,Anti-Integrin β2 Antibody, clone P4H9

merck millipore,默克密理博,14-906M,PI3K-C2α Protein, 250 µg merck millipore,默克密理博,14-906M,PI3K-C2α Protein, 250 µg

merck millipore,默克密理博,551826,QUIN 2, Tetrapotassium Salt - CAS 73630-23-6 - Calbiochem merck millipore,默克密理博,551826,QUIN 2, Tetrapotassium Salt - CAS 73630-23-6 - Calbiochem

merck millipore,默克密理博,AX45005PB,AXIS™ Axon Isolation Device, 450 µm, Plasma Bonded merck millipore,默克密理博,AX45005PB,AXIS™ Axon Isolation Device, 450 µm, Plasma Bonded

merck millipore,默克密理博,SF-999,GDF3; Human, Cyanine 5 RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-999,GDF3; Human, Cyanine 5 RNA Detection Probe,SmartFlare


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部