- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABC432 描述 Anti-LC3B Antibody Alternate Names - Microtubule-associated protein 1 light chain 3 beta
- Microtubule-associated proteins 1A/1B light chain 3B
- Autophagy-related protein LC3 B
- Autophagy-related ubiquitin-like modifier LC3 B
- MAP1 light chain 3-like protein 2
- MAP1A/MAP1B light chain 3 B
- MAP1A/MAP1B LC3 B
背景信息 MAP1LC3B, also known as Autophagy-related protein LC3 B, LC3B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, and encoded by the gene MAP1LC3B/MAP1ALC3, is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). MAP1LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. MAP1LC3B interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. MAP1LC3B interacts with FYCO1 (via C-terminus). MAP1LC3B interacts with TP53INP1 and TP53INP2, and MAP1LC3B interacts with TBC1D25. MAP1LC3B directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. MAP1LC3B interacts with ATG4B, MAPK15 and BNIP3 protein as well. MAP1LC3B is localized to the cytoplasm and cytoskeleton membranes, specifically endomembranes and cytoplasmic vesicles in the autophagosome. MAP1LC3B is expressed in most abundantly in heart, brain, skeletal muscle and testis tissues, with very low expression in liver tissues. Interestingly, high expression of MAP1LC3B in triple negative breast cancer (TNBC) is associated with poor outcomes in TNBC patients and is currently being studied as a prognostic evaluation marker for patients with TNBC. 产品信息 格式 Serum 演示 Rabbit polyclonal serum with 0.05% sodium azide. 应用 应用 Detect MAP1 using this rabbit polyclonal antibody, Anti-LC3B Antibody validated for use in western blotting. 主要应用 - Western Blotting
应用说明 Western Blotting Analysis: A representative lot from an independent laboratory detected LC3B in shRNA control HeLa cell lysates and demonstrated a loss of signal in TBC1D5 knockdown HeLa cell lysates. HeLa cells were either serum starved, or treated with bafilomycin A1 or serum starved in the presence of bafilomycin A1 (Popovic, D., et al. (2012). Mol Cell Biol. 32(9):1733-1744.). 生物信息 免疫原品种 Linear peptide corresponding to human LC3B. 宿主 Rabbit 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_073729
基因符号 - MAP1ALC3
- MAP1LC3B
纯化方法 Unpurified UniProt编号 - Q9GZQ8
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected LC3B in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,16-156,Protein A Agarose, Fast Flow
下一件merck millipore产品：merck millipore,默克密理博,4500-0560,Guava Caspase 9 FAM Kit for Flow Cytometry
相关产品
merck millipore,默克密理博,AM76,Anti-TBK1 Mouse mAb (108A429)
merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit
merck millipore,默克密理博,SCC058,FibroGRO™ Xeno-Free Human Foreskin Fibroblasts
merck millipore,默克密理博,2031,D-(+)-Biotin
merck millipore,默克密理博,16-156,Protein A Agarose, Fast Flow
merck millipore,默克密理博,4500-0560,Guava Caspase 9 FAM Kit for Flow Cytometry
merck millipore,默克密理博,OP107,Anti-BRCA1 (Ab-4) Mouse mAb (SD118)
merck millipore,默克密理博,07-107,Anti-Sp3 Antibody
merck millipore,默克密理博,PC16,Anti-FGF, Basic (Ab-2) (40-63) Rabbit pAb
merck millipore,默克密理博,362331,GAG Agonist, Adhesamine - Calbiochem