merck millipore,默克密理愽
  • merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit

  • merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit

    产品名称：Chromatin Immunoprecipitation (ChIP) Assay Kit
    产品型号：17-295
    Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

    merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit

  • 产品介绍
  • merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit

    重要规格表
    描述
    产品目录编号 17-295
    品牌系列 Upstate
    商名
    • Upstate
    描述 Chromatin Immunoprecipitation (ChIP) Assay Kit
    概述 For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.
    Alternate Names
    • Agarose ChIP Kit
    背景信息 Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
    产品信息
    部件
    • All necessary buffers
    • Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)
    演示 Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
    应用
    应用 Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.
    产品使用声明
    质量保证 Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit
    包装 Kit capacity: 22 Assays

    merck millipore,默克密理博,17-295,Chromatin Immunoprecipitation (ChIP) Assay Kit

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