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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB9610 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Olig-2 Antibody Alternate Names - Protein kinase C-binding protein RACK17
- Oligodendrocyte transcription factor 2
- Oligo2
- Class B basic helix-loop-helix protein 1
- bHLHb1
- Class E basic helix-loop-helix protein 19
- bHLHe19
- Protein kinase C-binding protein 2
背景信息 Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. The restricted expression of Olig2 in oligodendrocytes makes AB9610 an excellent marker of oligodendroglia and oligodendrogliomas in development and mature systems. 产品信息 格式 Purified 控制 - Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate
演示 Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. 应用 应用 Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western Blot Analysis:
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Optimal working dilutions must be determined by the end user生物信息 免疫原品种 Recombinant mouse Olig-2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the ~32 kDa Olig-2 protein by Western blot. 品种反应性 RatHumanMouse Species Reactivity Note Human, rat and mouse. Other species have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005806.2
Entrez基因汇总 This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. 基因符号 - Oligo2
- OLIG2
- bHLHB1
- RACK17
- OLIGO2
- BHLHB1
- PRKCBP2
纯化方法 Ammonium sulfate precipitation UniProt编号 - Q13516
UniProt汇总 FUNCTION: SwissProt: Q13516 # Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).
SIZE: 323 amino acids; 32385 Da
SUBUNIT: Interacts with NKX2-2 (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2 (By similarity).
TISSUE SPECIFICITY: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
DOMAIN:SwissProt: Q13516 The bHLH is essential for interaction with NKX2-2 (By similarity).DISEASE:SwissProt: Q13516 # A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. ...产品使用声明 质量保证 Evaluated by immunohistochemistry on glioblastoma
Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µL
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