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merck millipore,默克密理博,05-1328,Anti-PHD3 Antibody, clone 188e
产品名称：Anti-PHD3 Antibody, clone 188e
产品型号：05-1328
Anti-PHD3 Antibody, clone 188e is a mouse monoclonal antibody for detection of PHD3 also known as EGL nine (C.elegans) homolog 3, HIF-prolyl hydroxylase 3, Hypoxia-inducible factor prolyl hydroxylase More>>
merck millipore,默克密理博,05-1328,Anti-PHD3 Antibody, clone 188e
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1328 描述 Anti-PHD3 Antibody, clone 188e Alternate Names - egl nine-like protein 3 isoform
- EGL nine (C.elegans) homolog 3
- HIF prolyl hydroxylase 3
- HIF-prolyl hydroxylase 3
- Hypoxia-inducible factor prolyl hydroxylase 3
- Prolyl hydroxylase domain-containing protein 3
- egl nine homolog 3
- egl nine homolog 3 (C. elegans)
背景信息 PHD3 (Prolyl hydroxylase domain-containing protein 3), also known as Hypoxia-inducible factor prolyl hydroxylase 3 (HIF-PH3 or HPH-1), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. PHD3, is expressed in response to hypoxia and causes apoptosis in oxygenated conditions in neural cells. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 产品信息 格式 Purified 控制 - Human placenta tissue lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-PHD3 Antibody, clone 188e is a mouse monoclonal antibody for detection of PHD3 also known as EGL nine (C.elegans) homolog 3, HIF-prolyl hydroxylase 3, Hypoxia-inducible factor prolyl hydroxylase 3, & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to human PHD3. 表位 Unknown 克隆 188e 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PHD3. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_071356.1
基因符号 - PHD3
- EGLN3
- HIF-PH3
- HIFPH3
- HPH-1
纯化方法 Protein G purfied UniProt编号 - Q9H6Z9
UniProt汇总 FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism By similarity.
CATALYTIC ACTIVITY: An HIF alpha chain L-proline + 2-oxoglutarate + O2 = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO2.
COFACTOR: Binds 1 Fe2+ ion per subunit.
Ascorbate.
ENZYME REGULATION: Activated in cardiovascular cells and HelA cells following exposure to hypoxia.
SUBUNIT STRUCTURE: Interacts with MORG1 By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
SEQUENCE SIMILARITIES: Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.产品使用声明 质量保证 Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PHD3 on 10 µg of human placenta tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,SF-1201,miR-584-5p; Human, Cyanine 5 RNA Detection Probe,SmartFlare
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