merck millipore,默克密理博,MABF23,Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal

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merck millipore,默克密理博,MABF23,Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal
产品名称：Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal
产品型号：MABF23
This Anti-Indian Hedgehog (IHH) (N-term) Antibody, clone EP1192Y, Rabbit is validated for use in WB, IH(P), IP for the detection of Indian Hedgehog (IHH) (N-term).

merck millipore,默克密理博,MABF23,Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABF23,Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABF23
描述	Anti-Indian Hedgehog (IHH) (NT) Antibody, clone EP1192Y, rabbit monoclonal
Alternate Names	Indian hedgehog homolog Indian hedgehog (Drosophila) homolog Indian hedgehog Indian hedgehog homolog (Drosophila) indian hedgehog protein
背景信息	Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).

产品信息
格式	Unpurified
控制	Fetal brain lysate
演示	Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	This Anti-Indian Hedgehog (IHH) (N-term) Antibody, clone EP1192Y, Rabbit is validated for use in WB, IH(P), IP for the detection of Indian Hedgehog (IHH) (N-term).
主要应用	Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:250 – 1:500 dilution from a previous lot detected IHH in human fetal colon. Immunoprecipitation Analysis: A 1:30 dilution from a previous lot detected IHH by IP.

生物信息
免疫原品种	Synthetic peptide corresponding to human IHH near the N-terminus.
表位	N-terminus
克隆	EP1192Y
宿主	Rabbit
特异性	This antibody recognizes IHH near the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002172.2
Entrez基因汇总	This gene encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia.
基因符号	HHG-2 BDA1 HHG2
纯化方法	Unpurified
UniProt编号	Q14623
UniProt汇总	FUNCTION: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) By similarity. SUBCELLULAR LOCATION: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity. Indian hedgehog protein C-product: Secreted › extracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity. TISSUE SPECIFICITY: Expressed in embryonic lung, and in adult kidney and liver. PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity. Cholesterylation is required for N-product targeting to lipid rafts and multimerization By similarity. Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity. Ref.6 INVOLVEMENT IN DISEASE: Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short. Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles. SEQUENCE SIMILARITIES: Belongs to the hedgehog family.

产品使用声明
质量保证	Evaluated by Western Blot in fetal brain lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected IHH in fetal brain lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.