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merck millipore,默克密理博,04-1061,Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, rabbit monoclonal
产品名称：Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, rabbit monoclonal
产品型号：04-1061
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
merck millipore,默克密理博,04-1061,Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,04-1061,Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, rabbit monoclonal
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Unpurified Monoclonal Antibody 描述 产品目录编号 04-1061 描述 Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, rabbit monoclonal Alternate Names - neurofilament, light polypeptide 68kDa
- 68 kDa neurofilament protein
- Neurofilament triplet L protein
- light molecular weight neurofilament protein
- neurofilament protein, light chain
- neurofilament subunit NF-L
- neurofilament, light polypeptide
背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complex of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. 产品信息 格式 Unpurified 控制 - Untreated and treated SHSY5Y cell lysate.
演示 Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. 主要应用 - Western Blotting
生物信息 免疫原品种 Phospho-synthetic peptide corresponding to residues surrounding Serine 614/619 of human Neurofilament M. 表位 Phosphorylated Ser614/Ser619 克隆 EPR580(2)Y 宿主 Rabbit 特异性 This antibody recognizes phosphorylated Ser614/619 of Neurofilament M. 同种型 IgG 品种反应性 Human Species Reactivity Note Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001099011.1
Entrez基因汇总 Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. 基因符号 - neurofilament-light
- CMT1F
- CMT2E
- FLJ53642
- NF-L
- NF68
- NFL
修改 - Phosphorylation
纯化方法 Unpurified UniProt编号 - P07196
UniProt汇总 FUNCTION: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SUBUNIT STRUCTURE: Interacts with RGNEF By similarity.
DOMAIN: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
PTM: O-glycosylated By similarity.
INVOLVEMENT IN DISEASE: Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.产品使用声明 质量保证 Evaluated by Western Blot on untreated and treated SHSY5Y cell lysates.
Western Blot Analysis: 1:500 dilution of this antibody was used to detect Neurofilament M in untreated and treated with alkaline phosphatase SHSY5Y cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 100 µL
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