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merck millipore,默克密理博,AB951,Anti-Neuron Specific Enolase Antibody
产品名称：Anti-Neuron Specific Enolase Antibody
产品型号：AB951
Detect Neuron Specific Enolase using this Anti-Neuron Specific Enolase Antibody validated for use in IH, IH(P) & WB.
merck millipore,默克密理博,AB951,Anti-Neuron Specific Enolase Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B WB, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB951 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neuron Specific Enolase Antibody Alternate Names - Neural Enolase
- Enolase 2
- NSE
- gamma Enolase
背景信息 The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha'-enolase), neuron-specific enolase (NSE; gamma,gamma'-enolase, also known as 14-3-2 protein and NSP)(Grasso et al. 1977; Schmechel et al. 1979) and the short-lived heterodimer alpha, gamma'-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa (Schmechel et al. 1978), which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors (Marangos 1985). NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome (DeGiorgio et al. 1995; Yamazaki et al. 1995). 产品信息 格式 Serum 控制 - Brain tissue
演示 Rabbit antiSerum. Liquid in 0.1M NaCl. Contains 0.1% sodium azide. Dilute if necessary, with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used). 应用 应用 Detect Neuron Specific Enolase using this Anti-Neuron Specific Enolase Antibody validated for use in IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western Blot: 1:100-1:1,000 (ECL) On denatured Western blots of purified bovine NSE the antibody recognizes a single band at approximately 39 kDa. On Western blots of whole rat brain lysates (non-reduced) a more complex series of bands is observed ranging from 78/80 kDa (NSE homodimer) to 20 kDa (gamma-enolase degradation product).
Immunohistochemistry: 1:100-1:1,000 (ABC) The antibody has been used successfully on formaldehyde-fixed cryostat, Vibratome and de-paraffinized tissues. For indirect immunofluorescence procedures use at a dilution of 1:80-1:160. Dilute to working concentration with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used).
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB951
This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody) & Triton (must be optimized, start @ 0.1%).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).生物信息 免疫原品种 Synthetic peptide obtained from hNSE. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB951 stains many neuronal cell bodies and processes within the central and peripheral nervous system. 品种反应性 RatBovine 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001975.2
Entrez基因汇总 This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. 基因符号 - EC 4.2.1.11
- ENO2
- NSE
- Gamma-enolase
纯化方法 Unpurified UniProt编号 - P09104
UniProt汇总 FUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
SIZE: 434 amino acids; 47269 Da
SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 500 µL
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