- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ox, Ca, H, R, Sh, Fe IH(P), IF Rb Serum Polyclonal Antibody 描述 产品目录编号 AB940 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Growth Hormone Antibody Alternate Names - GH
背景信息 ANTIGEN:
Purified growth hormone产品信息 格式 Serum 演示 Liquid rabbit serum. Contains 0.1% sodium azide. 应用 应用 Anti-Growth Hormone Antibody detects level of Growth Hormone & has been published & validated for use in IF, IH(P). 主要应用 - Immunohistochemistry (Paraffin)
- Immunofluorescence
应用说明 Immunofluorescence and immunoperoxidase staining of formalin-fixed and paraffin-embedded sections of anterior pituitary glands. Cryostat sections of the same tissues can also be used.
Optimal working dilutions must be determined by the end user.
IMMUNOPEROXIDASE STAINING:
1. Incubate the section with antiserum diluted 1:1000 - 1:2,000 in PBS at 37°C for 30 minutes.
2. Wash for 15 minutes with PBS at room temperature and treat sequentially with Goat anti-Rabbit IgG and PAP complex at 37°C. Wash excess reagent from the section as described above.
3. Treat the section with a mixture of 3,3'-diaminobenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections may be dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE STAINING:
1. Same as Step 1 above.
2. Incubate the section with antiserum diluted 1:20-1:40 in PBS at 37°C for 30 minutes.
3. After washing off the excess antiserum with PBS, treat the section with fluorescein labeled Goat anti-Rabbit IgG for 30 minutes at 37°C. Wash the tissue with PBS and mount under glycerine-saline.生物信息 宿主 Rabbit 特异性 Gives a positive and specific immunostaining of growth hormone-containing cells. 品种反应性 OxCanineHumanRatSheepFeline 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_022562.2
- NM_000515.3
- NM_022559.2
- NM_022560.2
- NM_022561.2
Entrez基因汇总 The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. 基因符号 - hGH-N
- GH1
- GH
- GH-N
- GHN
UniProt编号 - P01241
UniProt汇总 FUNCTION: SwissProt: P01241 # Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
SIZE: 217 amino acids; 24847 Da
SUBUNIT: Monomer, dimer, trimer, tetramer and pentamer, disulfide- linked or non-covalently associated, in homopolymeric and heteropolymeric combinations. Can also form a complex either with GHBP or with the alpha2-macroglobulin complex.
SUBCELLULAR LOCATION: Secreted.
DISEASE: SwissProt: P01241 # Defects in GH1 are a cause of isolated growth hormone deficiency type IB (IGHD IB) [MIM:262400]; also known as pituitary dwarfism I. IGHD IB is an autosomal recessive deficiency of GH which causes short stature. & Defects in GH1 are the cause of Kowarski syndrome [MIM:262650]; also known as pituitary dwarfism VI. & Defects in GH1 are a cause of isolated growth hormone deficiency type II (IGHD II) [MIM:173100]. IGHD II is an autosomal dominant deficiency of GH which causes short stature. & Defects in GH1 may be a cause of short stature [MIM:604271]. Short stature is defined by a subnormal rate of growth.
SIMILARITY: SwissProt: P01241 ## Belongs to the somatotropin/prolactin family.
MISCELLANEOUS: Circulating GH shows a great heterogeneity due to alternative splicing, differential post-translational modifications of monomeric forms, oligomerization, optional binding to 2 different GH-binding proteins, and potentially proteolytic processing.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
上一件merck millipore产品：merck millipore,默克密理博,MABN536,Anti-Neuregulin-HBD, Type I/II Antibody, clone N120A/9
下一件merck millipore产品：merck millipore,默克密理博,07-1458,Anti-ROCK-1/2 Antibody
相关产品
merck millipore,默克密理博,218809,Caspase Substrate Set IV, Fluorogenic - Calbiochem
merck millipore,默克密理博,SF-132,HPRT1; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,05-23-5501,Parathyroid Hormone 1-34, Human - CAS 52232-67-4 - Calbiochem
merck millipore,默克密理博,MAB1388Z,Anti-Integrin β2 Antibody, clone YFC118.3, azide free
merck millipore,默克密理博,MABN536,Anti-Neuregulin-HBD, Type I/II Antibody, clone N120A/9
merck millipore,默克密理博,07-1458,Anti-ROCK-1/2 Antibody
merck millipore,默克密理博,559400,SB 203580, Iodo- - CAS 189319-35-5 - Calbiochem
merck millipore,默克密理博,AP112FH-K,Goat Anti-Human IgG H&L Fluor
merck millipore,默克密理博,AP112FH-K,Goat Anti-Human IgG H&L Fluor
merck millipore,默克密理博,AB19016,Anti-MMP-9 Antibody, Catalytic domain