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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB19016 Replaces 04-1150 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-9 Antibody, Catalytic domain Alternate Names - 92 kDa Type IV Collagenase
- Gelatinase B
背景信息 All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.产品信息 格式 Purified 控制 - HL60 cell lysate, U937 cell lysate Lung.
演示 Purified rabbit polyclonal in buffer containing PBS, containing 0.05% sodium azide. 应用 应用 Anti-MMP-9 Antibody, Catalytic domain is an antibody against MMP-9 for use in IH, IP & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western Blot Analysis:
1:1000 dilution of a previous lot was used.
Immunoprecipitation:
1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control.
Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) 表位 Catalytic domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes rat MMP-9 pro and active forms. Antibody also reacts with mouse MMP-9 and human MMP-9, other species not tested. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) as an immunogen. 品种反应性 RatHumanMouse Species Reactivity Note Rat, Human, and Mouse. Reactivity with other species has not been confirmed. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004985
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq] 基因符号 - EC 3.4.24.35 [Contains: 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9].
- MMP9
- MMP-9
- GELB
- CLG4B
纯化方法 Protein A purfied UniProt编号 - P14780
UniProt汇总 FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
SIZE: 707 amino acids; 78427 Da
SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.产品使用声明 质量保证 Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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