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品种反应性 主要应用 宿主 格式 抗体类型 H IH(P), IF Rb Serum Polyclonal Antibody 描述 产品目录编号 AB938 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Serotonin Antibody Alternate Names - 5-Hydroxytryptamine
- 5-HT
产品信息 格式 Serum 演示 Liquid antiserum. Contains 0.1% sodium azide. 应用 应用 Detect Serotonin using this Anti-Serotonin Antibody validated for use in IF, IH(P). 主要应用 - Immunohistochemistry (Paraffin)
- Immunofluorescence
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.生物信息 免疫原品种 The antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide. 宿主 Rabbit 特异性 The antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures. 品种反应性 Human 抗体类型 Polyclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
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