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merck millipore,默克密理博,AB3080P,Anti-Green Fluorescent Protein Antibody
产品名称:Anti-Green Fluorescent Protein Antibody
产品型号:AB3080P
Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB.
merck millipore,默克密理博,AB3080P,Anti-Green Fluorescent Protein Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, Vrt WB, ELISA, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3080P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Green Fluorescent Protein Antibody Alternate Names - eGFP
- GFP
背景信息 The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. 产品信息 格式 Affinity Purified 控制 - RENCX cells
- Cells transfected with GFP-tagged fusion vector
演示 Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer. 应用 应用 Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
应用说明 Western Blot:
A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.
ELISA:
A 1:800-1:1,000 dilution of a previous lot was used in ELISA.
Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore's highly purified secondary antibodies in indirect detection systems.
Immunocytochemistry:
Optimal working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Highly purified native GFP from Aequorea victoria. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli. 品种反应性 RatVertebrates Species Reactivity Note Most common vertebrate species tested. Reactivity with other species has not been confirmed. Expected to react with rat based on sequence homology. 抗体类型 Polyclonal Antibody 基因符号 - eGFP
- GFP
纯化方法 Affinity purified rabbit immunoglobulin UniProt编号 - P42212
产品使用声明 质量保证 Routinely evaluated by immunocytochemistry on RENCX cells.
Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot's anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.包装信息 数量 50 µg
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