- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 B, H, R, Po, Ca, Mk, Pm, Eq ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6000 Replaces AB802 描述 Anti-TIMP-3 Antibody, CT Alternate Names - Tissue inhibitor of metalloproteinases 3
- MIG-5
- TIMP metallopeptidase inhibitor 3
背景信息 Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix. 产品信息 格式 Affinity Purified 控制 - HL-60 cell lysate in PMA-conditioned media.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2. 品种反应性 BovineHumanRatPigCanineMonkeyPrimateHorse Species Reactivity Note Human and rat. Predicted to react with pig, canine, monkey, primate, equine, and bovine based on 100% sequence homology and mouse at 95% homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000353
Entrez基因汇总 This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq] 基因符号 - SFD
- TIMP3
- K222
- TIMP-3
纯化方法 Affinity Purfied UniProt编号 - P35625
UniProt汇总 FUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. May form part of a tissue- specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
SIZE: 211 amino acids; 24145 Da
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
DISEASE: Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.产品使用声明 质量保证 Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.
Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,SF-1620,miR-144-3p; Human, Cyanine 3 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,MABE133,Anti-CHD5 Antibody, clone 5A10
相关产品
merck millipore,默克密理博,CT02,MTT Cell Growth Assay Kit
merck millipore,默克密理博,AB2255,Anti-GABA B Receptor R2 Antibody
merck millipore,默克密理博,FCCH100110,FlowCellect Cytochrome C Kit
merck millipore,默克密理博,401442,Goat Anti-Human IgG, γ-Chain Specific Alkaline Phosphatase Conjugate
merck millipore,默克密理博,SF-1620,miR-144-3p; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MABE133,Anti-CHD5 Antibody, clone 5A10
merck millipore,默克密理博,AB5764,Anti-LMO4 Antibody
merck millipore,默克密理博,SF-180,miR-17-5p; Human Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,371830,GPR40 Antagonist, GW1100 - CAS 306974-70-9 - Calbiochem