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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, R, Pm WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB5895 Replaces AB5896 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-P2X3 Receptor Antibody, pain 产品信息 格式 Serum 演示 Rabbit serum. Liquid. Contains 0.05% sodium azide. 应用 应用 Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoblotting: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000
Immunocytochemistry: 1:500-1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5895
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRY
P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.生物信息 免疫原品种 Synthetic peptide corresponding to the C-terminus of rat P2X3. 宿主 Rabbit 特异性 P2X3
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.品种反应性 HumanRatPrimate 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002559.2
Entrez基因汇总 The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. 基因符号 - P2X3
- P2RX3
- MGC129956
UniProt编号 - P56373
UniProt汇总 FUNCTION: SwissProt: P56373 # Receptor for ATP that acts as a ligand gated ion channel.
SIZE: 397 amino acids; 44230 Da
SUBUNIT: Homo- or heteropolymers (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P56373 ## Belongs to the P2X receptor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. 包装信息 数量 50 µL
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