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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1963 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-N-WASP Antibody Alternate Names - Neural Wiskott-Aldrich Syndrome Protein
背景信息 Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain. 产品信息 格式 Affinity Purified 演示 Affinity-purified antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. 应用 应用 Anti-N-WASP Antibody is an antibody against N-WASP for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1:1000 ELISA: 1:2000 生物信息 免疫原品种 Synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP sequence. 宿主 Rabbit 特异性 This antibody detects a 65 kDa protein corresponding to the molecular weight of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa WASP protein in Jurkat cell lysate. 同种型 IgG 品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003941.2
Entrez基因汇总 The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. 基因符号 - NWASP
- WASL
- DKFZp779G0847
- MGC48327
- N-WASP
UniProt编号 - O00401
UniProt汇总 FUNCTION: SwissProt: O00401 # Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
SIZE: 505 amino acids; 54827 Da
SUBUNIT: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11 (By similarity). Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity). Nucleus (By similarity). Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity).
SIMILARITY: SwissProt: O00401 ## Contains 1 CRIB domain. & Contains 1 WH1 domain. & Contains 2 WH2 domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C for up to one year from date of receipt. Do not aliquot. 包装信息 数量 100 µL
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