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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ICC, IHC Gp Serum Polyclonal Antibody 描述 产品目录编号 AB5894 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-P2X2 Receptor Antibody 产品信息 格式 Serum 演示 Serum. Liquid. Contains 0.05% sodium azide. 应用 应用 Anti-P2X2 Receptor Antibody is an antibody against P2X2 Receptor for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoblotting: 1:500
Immunohistochemistry: 1:500.
Immunocytochemistry: 1:500.
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR AB5894
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5894 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRYP
2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above
WESTERN BLOTTING
Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with AB5894 diluted 1:500 with same buffer overnight at 2-8°C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence.生物信息 免疫原品种 A 13 amino acid peptide corresponding to amino acids 460-472 from the carboxy-terminus of the rat P2X2 Receptor protein.
Control peptide: Catalog Number AG354宿主 Guinea Pig 特异性 P2X2 Receptor. 品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_174873.1
- NM_012226.3
- NM_016318.2
- NM_170682.2
- NM_170683.2
- NM_174872.1
Entrez基因汇总 The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene. 基因符号 - P2X2
- P2RX2
- MGC129601
UniProt编号 - Q9UBL9
UniProt汇总 FUNCTION: SwissProt: Q9UBL9 # Binding of this ligand gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle.
SIZE: 471 amino acids; 51754 Da
SUBUNIT: Homo- or heteropolymers.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q9UBL9 ## Belongs to the P2X receptor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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