merck millipore,默克密理博,AB5786,Anti-Zic1 Antibody, near CT

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merck millipore,默克密理博,AB5786,Anti-Zic1 Antibody, near CT
产品名称：Anti-Zic1 Antibody, near CT
产品型号：AB5786
Anti-Zic1 Antibody, near C-terminus detects level of Zic1 & has been published & validated for use in WB, IH.

merck millipore,默克密理博,AB5786,Anti-Zic1 Antibody, near CT

产品介绍

merck millipore,默克密理博,AB5786,Anti-Zic1 Antibody, near CT

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5786
Replaces	MABE487
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Zic1 Antibody, near CT

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-Zic1 Antibody, near C-terminus detects level of Zic1 & has been published & validated for use in WB, IH.
主要应用	Immunohistochemistry Western Blotting
应用说明	Western blot: 1:200-1:1,000 using ECL. (48 kDa) Immunohistochemistry: 1:200-1:1,000. Staining will be predominantly nuclear. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide corresponding to amino acids 411-424 of Human Zic1 (SPSTDNPTTSSLSP).
表位	near C-terminus
宿主	Rabbit
特异性	Recognizes Zic1 (Odd-paired related), a zinc finger transcription factor.
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_003412.3
Entrez基因汇总	This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene.
基因符号	ZIC ZIC1 ZNF201
UniProt编号	Q15915
UniProt汇总	FUNCTION: SwissProt: Q15915 # May play a role in cerebellar development. SIZE: 447 amino acids; 48295 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined. SIMILARITY: SwissProt: Q15915 ## Belongs to the GLI C2H2-type zinc-finger protein family. & Contains 5 C2H2-type zinc fingers.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.