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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5776 Replaces AB9090 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Sox11 Antibody, pain 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. 应用 应用 Anti-Sox11 Antibody, pain is an antibody against Sox11 for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1:200-1:1,000 using ECL.
Immunohistochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide, amino acids 115-131 from human Sox-11. 宿主 Rabbit 特异性 Recognizes Sox-11, a high mobility group (HMG) transcription factor. 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003108.3
Entrez基因汇总 This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis. 基因符号 - SOX11
UniProt编号 - P35716
UniProt汇总 FUNCTION: SwissProt: P35716 # Probably important in the developing nervous system.
SIZE: 441 amino acids; 46679 Da
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Expressed mainly in the nervous system.
SIMILARITY: SwissProt: P35716 ## Contains 1 HMG box DNA-binding domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
PREPARATION AND USE:
To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.
For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.包装信息 数量 100 µg
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