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品种反应性 主要应用 宿主 格式 抗体类型 A WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB5411 Replaces AB5386 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nitrotyrosine Antibody 背景信息 The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten. 产品信息 格式 Purified 控制 - POSITIVE CONTROL: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite (catalog # 20-107) to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10 microliters of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
演示 Purified rabbit polyclonal in buffer containing 0.1% sodium azide. 应用 应用 Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody validated for use in IH(P) & WB. 主要应用 - Western Blotting
应用说明 Western Blot:
1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin.
Immunohistochemistry:
1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Nitrated KLH. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes nitrated tyrosine residues. 品种反应性 All Species Reactivity Note Human and mouse. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody 纯化方法 Protein A purfied 产品使用声明 质量保证 Immunohistochemistry(paraffin) Analysis:
Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus.
Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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