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merck millipore,默克密理博,208730,Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)
产品名称:Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)
产品型号:208730
This Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Calpain-1/2, Small Subunit.
merck millipore,默克密理博,208730,Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain. 产品目录编号 208730 品牌系列 Calbiochem®
应用数据 
This antibody can be used to immunoprecipitate the small subunit of Calpain I and Calpain II under native and denatured conditions. Immunoprecipitation (10% SDS-PAGE) Samples: A431 cells lysed under native conditions and immunoprecipitated with clone 28F3 anti-calpain I,II small subunit (Cat. No. 208730) (lane 1), an irrelevant antibody (lane 2) and direct lysate (lane 3). Primary antibody concentration: 0.5 µg/ml (Cat. No: 208730) Secondary antibody concentration: 0.2 µg/ml goat anti-mouse IgG-HRP, γ-chain specific (Cat. No. 401207) Exposure: 1 min Obtained results: Bands at ~30 kDa in lanes 1 and 3产品信息 形式 Liquid 配方 In 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5. 正控制 MDBK cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 purified calpain from human placenta 免疫原品种 Human Placenta 克隆 28F3 宿主 Mouse 同种型 IgG₁ 品种 bovine, human, not mouse, not rat 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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