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  • merck millipore,默克密理博,AB4334,Anti-NODAL Antibody

  • merck millipore,默克密理博,AB4334,Anti-NODAL Antibody

    产品名称：Anti-NODAL Antibody
    产品型号：AB4334
    Detect NODAL using this Anti-NODAL Antibody validated for use in WB, IH(P) & IC.

    merck millipore,默克密理博,AB4334,Anti-NODAL Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB4334,Anti-NODAL Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Rb, M, H, Po, Eq, Chp, Rhesus Macaque, RICC, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB4334
    描述 Anti-NODAL Antibody
    Alternate Names
    • nodal, mouse, homolog
    • nodal homolog (mouse)
    背景信息 NODAL is a highly conserved, secreted homodimer belonging to the TGF-beta family of proteins involved in developmental regulation. One of the most prominent, endogenous molecules observed at this early stage, NODAL stimulates the formation of both the endoderm and mesoderm, designates the embryonic axis, and is necessary to the asymmetrical development of organs such as the brain, heart, and visceral organs. It is also a crucial component for embryonic stem (ES) cell differentiation into specific tissue lineages. In cooperation with activin, NODAL signaling has been shown to play a role in maintaining ES cell pluripotency by regulating Nanog expression.
    产品信息
    格式 Affinity Purified
    控制
    • Mouse E7 fetus tissue lysate
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Detect NODAL using this Anti-NODAL Antibody validated for use in WB, IH(P) & IC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Western Blot (Snap i.d.®) Analysis: 5 µg/mL of this antibody detected NODAL in 10 µg of mouse E7 fetus tissue lysate.

    Immunohistochemistry Analysis: A 1:300 dilution from a previous lot detected NODAL in metastatic endometrial carcinoma and colorectal carcinoma tissue.

    Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected NODAL in mouse embryonic stem cells.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of the mature chain of mouse NODAL.
    表位 N-terminus of mature chain
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes NODAL near the N-terminus of the mature chain.
    品种反应性 RabbitMouseHumanPigHorseChimpanzeeRhesus MacaqueRat
    Species Reactivity Note Demonstrated to react with mouse and human.
    Predicted to react with porcine, equine, chimpanzee, rhesus macaque, rat, and rabbit based on 100% sequence homology.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_038639
    基因符号
    • Nodal
    • NODAL
    纯化方法 Affinity Purified
    UniProt编号
    • P43021
    UniProt汇总 FUNCTION: Essential for mesoderm formation and axial patterning during embryonic development.

    SUBUNIT STRUCTURE: Homodimer; disulfide-linked (By similarity).

    SUBCELLULAR LOCATION: Secreted (By similarity).

    DEVELOPMENTAL STAGE: Expressed in the node during gastrulation. Expression is first detected in primitive streak-stage embryos at about the time of mesoderm formation. It then becomes highly localized in the node at the anterior of the primitive streak.

    SEQUENCE SIMILARITIES: Belongs to the TGF-beta family.
    产品使用声明
    质量保证 Evaluated by Western Blot in mouse E7 fetus tissue lysate.

    Western Blot Analysis: 0.5 µg/mL of this antibody detected NODAL in 10 µg of mouse E7 fetus tissue lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,AB4334,Anti-NODAL Antibody

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