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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2994 描述 Anti-Phospho-Bid (Ser65) Antibody Alternate Names - p22 BID
- BH3 interacting domain death agonist
- BH3-interacting domain death agonist
- BID isoform ES(1b)
- BID isoform L(2)
- BID isoform Si6
- Human BID coding sequence
- apoptic death agonist
- desmocollin type 4
背景信息 Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release. 产品信息 格式 Affinity Purified 控制 - Western Blot:: Jurkat cell lysate
演示 Purified in PBS with 0.09% NaN3. 应用 应用 Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB. 主要应用 - Western Blotting
生物信息 表位 Ser65 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody reacts with the 22 kDa phospho-Bid-S65 protein. 品种反应性 Human Species Reactivity Note Reacts with human. Other species have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001196.2
Entrez基因汇总 This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]
基因符号 - OTTHUMP00000196197
- FP497
- MGC15319
- MGC42355
修改 - Phosphorylation
纯化方法 Antigen affinity purified UniProt编号 - P55957-2
UniProt汇总 FUNCTION:The major proteolytic product p15 BID allows the release of cytochrome c By similarity. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SUBUNIT STRUCTURE: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 By similarity.
SUBCELLULAR LOCATION:CytoplasmBy similarity. Mitochondrion membraneBy similarity. Note= When uncleaved, it is predominantly cytoplasmic.
BH3-interacting domain death agonist p15: Mitochondrion membraneBy similarity. Note= Translocates to mitochondria as an integral membrane protein By similarity.
BH3-interacting domain death agonist p13: Mitochondrion membraneBy similarity. Note= Associated with the mitochondrial membrane By similarity.
Isoform 1: Cytoplasm. Ref.3
Isoform 3: Cytoplasm. Ref.3
Isoform 2: Mitochondrion membrane. Note= A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. Ref.3
TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). Ref.3
DOMAIN:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products By similarity.
Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.10
产品使用声明 质量保证 Evaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.
Western Blot Analysis:
A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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