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merck millipore,默克密理博,AB3914,Anti-SMC3 Antibody
产品名称：Anti-SMC3 Antibody
产品型号：AB3914
Use Anti-SMC3 Antibody (Rabbit Polyclonal Antibody) validated in IP, WB, ICC to detect SMC3 also known as Structural Maintanence of Chromosomes 3, Chondroitin Sulfate Proteoglycan 6.
merck millipore,默克密理博,AB3914,Anti-SMC3 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3914 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-SMC3 Antibody Alternate Names - hCAP
- Structural Maintanence of Chromosomes 3
- Chondroitin Sulfate Proteoglycan 6
- Bamacan
背景信息 Structural Maintenance of Chromomosomes-3 (SMC3) protein is involved in chromomosome cohesion during cell cycle and DNA repair. This nuclear protein is a key component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. 应用 应用 Use Anti-SMC3 Antibody (Rabbit Polyclonal Antibody) validated in IP, WB, ICC to detect SMC3 also known as Structural Maintanence of Chromosomes 3, Chondroitin Sulfate Proteoglycan 6. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot: 1:1,000-1:10,000
Immunocytochemistry: 1:200-1:800
Immunoprecipitation: 2-6 μL
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from human SMC-3. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Structural Maintenance of Chromosomes-3 (SMC-3). 品种反应性 XenopusHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005445.3
Entrez基因汇总 This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. 基因符号 - hCAP
- SMC3
- bamacan
- BMH
- HCAP
- BAM
- CDLS3
- CSPG6
- SMC3L1
- Bamacan
UniProt编号 - Q9UQE7
UniProt汇总 FUNCTION: SwissProt: Q9UQE7 # Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.
SIZE: 1217 amino acids; 141542 Da
SUBUNIT: Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement.
SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
DOMAIN: SwissProt: Q9UQE7 The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: Q9UQE7 # Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
SIMILARITY: Belongs to the SMC family. SMC3 subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted for up to 6 months. 包装信息 数量 50 µg
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