merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3849,Anti-Smad2 Antibody, phospho-specific (Ser465/467)

  • merck millipore,默克密理博,AB3849,Anti-Smad2 Antibody, phospho-specific (Ser465/467)

    产品名称：Anti-Smad2 Antibody, phospho-specific (Ser465/467)
    产品型号：AB3849
    Use Anti-Smad2 Antibody, phospho-specific (Ser465/467) (Rabbit Polyclonal Antibody) validated in ICC, IF, IHC(P), WB to detect Smad2 also known as MAD-mothers against decapentaplegic-homolog 2.

    merck millipore,默克密理博,AB3849,Anti-Smad2 Antibody, phospho-specific (Ser465/467)

  • 产品介绍
  • merck millipore,默克密理博,AB3849,Anti-Smad2 Antibody, phospho-specific (Ser465/467)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3849
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Smad2 Antibody, phospho-specific (Ser465/467)
    Alternate Names
    • mother against DPP homolog 2
    • MAD, mothers against decapentaplegic homolog 2 (Drosophila)
    • Mad protein homolog 2
    • Mad, mothers against decapentaplegic homolog 2
    • Mad-related protein 2
    • Mothers against DPP homolog 2
    • SMAD 2
    • SMAD family member 2
    • SMAD, mothers against DPP homolog 2
    • SMAD, mothers against DPP homolog 2 (Drosophila)
    • Sma- and Mad-related protein 2
    • mothers against DPP homolog 2
    • mothers against DPP homolog 2 (Drosophila)
    • Sma- and Mad-related protein 2
    背景信息 SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-β superfamily of modulators. Like many other TGF-β family members SMAD2 is involved in cell signaling. SMAD2 modulates signals of activin and TGF-β's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
    产品信息
    格式 Affinity Purified
    控制
    • POSITIVE CONTROL: TGF-β treated HepG2 cells.
    演示 Purified rabbit polyclonal in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol.
    应用
    应用 Use Anti-Smad2 Antibody, phospho-specific (Ser465/467) (Rabbit Polyclonal Antibody) validated in ICC, IF, IHC(P), WB to detect Smad2 also known as MAD-mothers against decapentaplegic-homolog 2.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    应用说明 Western Blotting:
    1:1,000 dilution of a previous lot was used. For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C overnight with gentle agitation.

    Immunohistochemistry (Paraffin):
    1:2000 dilution of a previous lot was used.

    Immunocytochemistry (IF):
    1:500 dilution of a previous lot was used.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
    表位 Phospho-Ser465/467
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 By western blot, the antibody recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The antibody may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not cross-react with other Smad-related proteins
    品种反应性 RatHumanMouse
    Species Reactivity Note Human, mouse and rat. The antibody is predicted to react with chicken, Xenopus, and zebrafish due to 100% sequence similarity.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_001003652.2
    Entrez基因汇总 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
    基因符号
    • JV18-1
    • SMAD2
    • hMAD-2
    • JV18
    • hSMAD2
    • MGC22139
    • MADH2
    • MGC34440
    • MADR2
    修改
    • Phosphorylation
    纯化方法 Protein A and peptide affinity purified immunoglobulin
    UniProt编号
    • Q15796
    UniProt汇总 FUNCTION: SwissProt: Q15796 # Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
    SIZE: 467 amino acids; 52306 Da
    SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2- alpha subunit, CREB-binding protein (CBP), p300, SKI, SNON and SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity).
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    TISSUE SPECIFICITY: Expressed at high levels in skeletal muscle, heart and placenta.
    PTM: Phosphorylated on one or several of Thr-220, Ser-245, Ser- 250, and Ser-255. In response to TGF-beta, phosphorylated on Ser- 465/467 by TGF-beta and activin type 1 receptor kinases. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. & In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).
    DISEASE: SwissProt: Q15796 # Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.
    SIMILARITY: SwissProt: Q15796 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB3849,Anti-Smad2 Antibody, phospho-specific (Ser465/467)

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