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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3276-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 3 Antibody Alternate Names - GLIP
- AQP3
产品信息 格式 Affinity Purified 控制 - Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). 应用 应用 Detect Aquaporin 3 using this Anti-Aquaporin 3 Antibody validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blotting: 1:200 on rat kidney membranes. Identifies a 31kDa and 40kDa band. The large band may represent the glycosylated form of the protein.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Highly purified peptide corresponding to residues 275-292 of rat Aquaporin 3 (Accession Number P47862) coupled to KLH. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Aquaporin 3. 品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004925.3
Entrez基因汇总 Aquaporin 3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. 基因符号 - GIL
- AQP3
- AQP-3
- Aquaporin-3
纯化方法 Immunoaffinity purified UniProt编号 - Q92482
UniProt汇总 FUNCTION: SwissProt: Q92482 # Forms a water-specific channel that provide kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. May function as a water and urea exit mechanism in antidiuresis in collecting duct cells. Also slightly permeable to urea and glycerol. May play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
SIZE: 292 amino acids; 31544 Da
SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein. Note=In collecting ducts of kidney.
TISSUE SPECIFICITY: Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
DOMAIN:SwissProt: Q92482 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
上一件merck millipore产品：merck millipore,默克密理博,MABT44,Anti-ICAM-3 (CD50) Antibody, clone CBR-IC3/1
下一件merck millipore产品：merck millipore,默克密理博,SF-3007,CRP; Human, Cyanine 3 RNA Detection Probe,SmartFlare
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