merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3260-50UL,Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

  • merck millipore,默克密理博,AB3260-50UL,Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

    产品名称：Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT
    产品型号：AB3260-50UL
    Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, C-terminus is an antibody against Endothelin Receptor A for use in IC, IH & WB.

    merck millipore,默克密理博,AB3260-50UL,Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

  • 产品介绍
  • merck millipore,默克密理博,AB3260-50UL,Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    RWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3260-50UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT
    Alternate Names
    • ET-A
    产品信息
    格式 Affinity Purified
    控制
    • Included free of charge with the antibody is 30 μg of control antigen (lyophilized). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, C-terminus is an antibody against Endothelin Receptor A for use in IC, IH & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry on rat brain sections.

    Western blot: 2.5 μg/mL (1:200) using ECL on rat brain membranes.

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.

    SUGGESTED WESTERN BLOT PROTOCOL

    1. Mix the samples with sample-buffer X 2, and heat 10 min at 70°C.

    2. 5-50 mL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h)

    3. Transfer in semi-dry system under standard conditions (3 h 200 mA for two minigel gels)

    4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

    5. Destain with deionized water.

    6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

    7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

    8. Wash 4 x 15 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

    9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

    10. Wash 4 x 15 min. with PBS-0.1% tween 20.

    11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).
    生物信息
    免疫原品种 Purified peptide corresponding to amino acids 413-426 of the rat Endothelin Receptor A (Accession P26684).
    表位 a.a. 413-426 rET-A, C-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Endothelin Receptor A (ET-A). The immunogen sequence is specific for ET-A and is not present in any other known protein. The antibody is not expected to cross with ET-B but it has not been specifically tested.

    SPECIES REACTIVITIES: The immunogen sequence is highly conserved in human, porcine, bovine (13/14 residues) and chicken (12/14). Reactivity with other species has not been confirmed.
    品种反应性 Rat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_001957.1
    基因符号
    • ETA-R
    • EDNRA
    • ETRA
    • ETA
    • ET-A
    UniProt编号
    • P25101
    UniProt汇总 FUNCTION: SwissProt: P25101 # Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 > ET3.
    SIZE: 427 amino acids; 48722 Da
    SUBUNIT: Interacts with HDAC7 and HTATIP.
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
    SIMILARITY: SwissProt: P25101 ## Belongs to the G-protein coupled receptor 1 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB3260-50UL,Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

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