merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3201,Anti-Tubulin Antibody, Detyrosinated

  • merck millipore,默克密理博,AB3201,Anti-Tubulin Antibody, Detyrosinated

    产品名称：Anti-Tubulin Antibody, Detyrosinated
    产品型号：AB3201
    Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) & WB.

    merck millipore,默克密理博,AB3201,Anti-Tubulin Antibody, Detyrosinated

  • 产品介绍
  • merck millipore,默克密理博,AB3201,Anti-Tubulin Antibody, Detyrosinated

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Su, H, M, R, Ma, PlWB, ICCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3201
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Tubulin Antibody, Detyrosinated
    Alternate Names
    • Glu Tubulin
    背景信息 Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
    产品信息
    格式 Affinity Purified
    控制
    • Hela Whole Cell Lysate.
    演示 Purified rabbit polyclonal in buffer containing liquid PBS.
    应用
    应用 Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunohistochemistry:
    A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Peptide corresponding to the C-terminal of Glu tubulin.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).
    品种反应性 Sea UrchinHumanMouseRatMammalsGreen Plants
    Species Reactivity Note Human, mouse, and rat. Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_006086.2
    基因符号
    • beta-4
    • TUBB3
    • TUBB4
    • MC1R
    非反应性品种
    • Yeast (S. cerevisiae)
    纯化方法 Affinity purified immunoglobulin
    UniProt编号
    • Q13509
    UniProt汇总 FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    SIZE: 450 amino acids; 50433 Da
    SUBUNIT: Dimer of alpha and beta chains.
    DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
    SIMILARITY: Belongs to the tubulin family.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on L6 lysate lysates.

    Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at -20°C in undiluted aliquots from date of receipt.

    Handling Recommendations:
    Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB3201,Anti-Tubulin Antibody, Detyrosinated

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