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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB3116 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bcl-xL Antibody, Phospho-specific 背景信息 Bcl-XL is one of the anti-apoptotic members of the Bcl-2 family of apoptosis regulators and is overexpressed in many human cancers. Studies by Dr. Haldar and his associates suggest that microtubule damaging drugs like Taxol or 2-methoxyestradiol induce phosphorylation of Bcl-XL at serine 62 by kinases such as Jun and this allows those cells to die by apoptosis. 产品信息 格式 Serum 演示 Liquid rabbit serum containing no preservatives. 应用 应用 Detect Bcl-xL using this Anti-Bcl-xL Antibody, Phospho-specific validated for use in WB. 主要应用 - Western Blotting
应用说明 Immunoblotting: Recommended dilution is 1:100
Optimal dilutions must be determined by end-user.生物信息 免疫原品种 Synthetic phosporylated peptide corresponding to a sequence encompassing serine 62 of human Bcl-xL conjugated to KLH. 表位 Phospho-specific 宿主 Rabbit 特异性 AB3116 is specific for the Bcl-xL phosphorylated on serine 62. It does not recognize the unphosphorylated form of the protein. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_138578.1
- NM_001191.2
Entrez基因汇总 The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. 基因符号 - bcl-xS
- BCL2L1
- DKFZp781P2092
- BCL-XL/S
- BCLX
- bcl-xL
- Bcl-X
- BCL2L
修改 - Phosphorylation
UniProt编号 - Q07817
UniProt汇总 FUNCTION: SwissProt: Q07817 # Potent inhibitor of cell death. Isoform Bcl-X(L) anti- apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
SIZE: 233 amino acids; 26049 Da
SUBUNIT: Bcl-X(L) forms homodimers, and heterodimers with BAX, BAK and BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Also interacts with BAD and BBC3. Isoform Bcl-X(L) binds to Siva isoform 1. Interacts with BCL2L11 (By similarity). Interacts with BECN1 and PGAM5.
SUBCELLULAR LOCATION: Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus envelope; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
TISSUE SPECIFICITY: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
DOMAIN: SwissProt: Q07817 The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
PTM: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
SIMILARITY: Belongs to the Bcl-2 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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