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品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA, ICC, IHC Ch Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3091 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 9 Antibody Alternate Names - AQP9
背景信息 Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.
A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.产品信息 格式 Affinity Purified 演示 Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA. 应用 应用 Detect Aquaporin 9 using this Anti-Aquaporin 9 Antibody validated for use in ELISA, IC, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1-10 μg/mL using ECL
ELISA: 1:100,000 using 50-100 ng of control peptide per well.
Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 An 18 AA synthetic peptide within the C-terminal domain of rat Aquaporin-9 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 Aquaporin 9. The immunogen peptide is unique to Aquaporin-9 without significant homology to any other Aquaporins.
Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_020980.3
Entrez基因汇总 The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. 基因符号 - SSC1
- AQP9
- HsT17287
- Aquaporin-9
- AQP-9
UniProt编号 - O43315
UniProt汇总 FUNCTION: SwissProt: O43315 # Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea but not to glycerol.
SIZE: 295 amino acids; 31401 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.
DOMAIN: SwissProt: O43315 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,14-314M,Syk Protein, active, 250 µg
下一件merck millipore产品：merck millipore,默克密理博,SLHV033RB,Millex-HV 过滤器，0.45 µm，PVDF，33 mm，可 γ 射线灭菌，已灭菌
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