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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2273 描述 Anti-ZO-2 Antibody Alternate Names - Tight junction protein 2
- tight junction protein 2 (zona occludens 2)
- tight junction protein ZO 2
- tight junction protein ZO-2
- Zona occludens protein 2
- Friedreich ataxia region gene X104 (tight junction protein ZO-2)
- Zonula occludens protein 2
背景信息 Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ZO-2 Antibody is an antibody against ZO-2 for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected ZO-2 on 10 µg of HEPG2 cell lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected ZO-2 in stomach cancer and colorectal carcinoma tissue.生物信息 免疫原品种 Linear peptide corresponding to the PDZ3 domain of human ZO-2. 表位 PDZ3 domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes ZO-2 at the PDZ3 domain. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Predicted to react with canine (dog) and chimpanzee based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004808
Entrez基因汇总 This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutation in this gene have been identified in patients with hypercholanemia. Alternate splicing results in multiple transcript variants. 基因符号 - TJP2
- ZO-2
- ZO2
纯化方法 Affinity purified UniProt编号 - Q9UDY2
UniProt汇总 FUNCTION: Plays a role in tight junctions and adherens junctions.
SUBUNIT STRUCTURE: Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus. Interacts with SCRIB.
SUBCELLULAR LOCATION: Cell junction › adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cell junction › tight junction (By similarity). Nucleus (By similarity). Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures (By similarity).
TISSUE SPECIFICTY: This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
INVOLVEMENT IN DISEASE: Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Ref.26
SEQUENCE SIMILARITIES: Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
SEQUENCE CAUTION: The sequence AAA61300.1 differs from that shown. Reason: Frameshift at positions 1086, 1092 and 1095.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected ZO-2 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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