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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2272 描述 Anti-ZO-1 Antibody Alternate Names - zona occludens 1
- tight junction protein 1
- zonula occludens 1 protein
- Zona occludens protein 1
- Zonula occludens protein 1
背景信息 Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. 产品信息 格式 Affinity Purified 控制 - HepG2 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect ZO-1 using this Anti-ZO-1 Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected ZO-1 in normal human testis tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human ZO-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Other homologies: Rat ( 89% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003248
Entrez基因汇总 This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. 基因符号 - TJP1
- ZO-1
- ZO1
纯化方法 Affinity purified UniProt编号 - Q07157
UniProt汇总 FUNCTION: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
SUBUNIT STRUCTURE: Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1 By similarity. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with occludin, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › tight junction. Note: Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
TISSUE SPECIFICTY: The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
DOMAIN: The second PDZ domain mediates interaction with GJA12 (By similarity).
SEQUENCE SIMILARITIES: Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain.
SEQUENCE CAUTION: The sequence AAA02891.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.产品使用声明 质量保证 Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ZO-1 on 10 µg of HepG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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