merck millipore,默克密理博,S7814,CpG WIZ RARβ2

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Merck Millipore 默克密理愽

merck millipore,默克密理博,S7814,CpG WIZ RARβ2 - Methylation specific PCR assay

描述
产品目录编号	S7814
品牌系列	Chemicon®
商名	Chemicon CpG Wiz
描述	CpG WIZ® RARβ2 - Methylation specific PCR assay
概述	Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). The Retinoic Acid Receptor beta2 is expressed at reduced levels in many tumor types as a result of hypermethylation of its promoter region. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

产品信息
部件	Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C The components of the CpG WIZ® RARbeta2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set7.5 μM each primer (25X) 35 μL (clear cap) 90595 -15°C to -25°C M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90596 -15°C to -25°C W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90597 -15°C to -25°C U control DNA0.1 μg/μL 50 μL (clear cap) 90393 -15°C to -25°C M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

产品信息

部件

Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
The components of the CpG WIZ® RARbeta2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
U Primer Set7.5 μM each primer (25X) 35 μL (clear cap) 90595 -15°C to -25°C
M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90596 -15°C to -25°C
W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90597 -15°C to -25°C
U control DNA0.1 μg/μL 50 μL (clear cap) 90393 -15°C to -25°C
M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

应用
应用	The components of the CpG WIZ RARbeta2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
主要应用	PCR
应用说明	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® RARbeta2 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

生物信息
品种反应性	Human
Entrez基因编号	NM_021976.3
Entrez基因汇总	This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
基因符号	DAUDI6 RXRB H-2RIIBP NR2B2 MGC1831 RCoR-1
UniProt编号	P28702
UniProt汇总	FUNCTION: SwissProt: P28702 # Nuclear hormone receptor. Involved in the retinoic acid response pathway. Binds 9-cis retinoic acid (9C-RA). SIZE: 533 amino acids; 56922 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in a variety of tumor cell lines. DOMAIN: SwissProt: P28702 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. SIMILARITY: Belongs to the nuclear hormone receptor family. NR2 subfamily. & Contains 1 nuclear receptor DNA-binding domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.