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品种反应性 主要应用 宿主 格式 抗体类型 M IHC, ELISA, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2033 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Fibronectin Antibody 背景信息 Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay. 产品信息 格式 Affinity Purified 控制 - Extracellular matrix in most tissue
演示 Purified rabbit polyclonal in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2 with no preservatives. 应用 应用 Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA. 主要应用 - Immunohistochemistry
- ELISA
- Immunocytochemistry
应用说明 Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.
Radioimmunoassay:
A previous lot of this antibody was used in RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Fibronectin extracted and purified from mouse plasma. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay. 品种反应性 Mouse Species Reactivity Note Mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002026.2
Entrez基因汇总 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. 基因符号 - FINC
- FN1
- MSF
- FN
- CIG
- LETS
纯化方法 Immunoaffinity purified UniProt编号 - P02751
UniProt汇总 FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains产品使用声明 质量保证 Evaluated by immunocytochemistry on NIH/3T3 cells.
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488® -Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive staining is restricted to the cytosol.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MABF297,Anti-RIG-I, clone 1C3 Antibody
下一件merck millipore产品：merck millipore,默克密理博,DET-HP1000,IHC Select HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Prediluted
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