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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1922 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin β4 Antibody Alternate Names - CD104
产品信息 格式 Serum 演示 Whole rabbit serum. Liquid, with 0.02% sodium azide. 应用 应用 Anti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH. 主要应用 - Immunohistochemistry
应用说明 Immunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.生物信息 免疫原品种 Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid 宿主 Rabbit 特异性 Specific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001005731.1
- NM_000213.3
- NM_001005619.1
Entrez基因汇总 Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. 基因符号 - GP150
- ITGB4
- CD104
UniProt编号 - P16144
UniProt汇总 FUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
SIZE: 1822 amino acids; 202151 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The undiluted antibody solution is stable for approximately 6 months when stored -20°C. 包装信息 数量 100 µL
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