merck millipore,默克密理博,17-10090,SIRTainty™ Class III HDAC Assay

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merck millipore,默克密理博,17-10090,SIRTainty™ Class III HDAC Assay
产品名称：SIRTainty™ Class III HDAC Assay
产品型号：17-10090
Class III HDAC assay used for the detection sirtuin activity levels. Assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a sub More>>

merck millipore,默克密理博,17-10090,SIRTainty™ Class III HDAC Assay

产品介绍

merck millipore,默克密理博,17-10090,SIRTainty™ Class III HDAC Assay

重要规格表

主要应用
EA

描述
产品目录编号	17-10090
商名	Upstate SIRTainty
描述	SIRTainty™ Class III HDAC Assay
概述	Features and Benefits Sensitive Detection – assay design enables detection of all known sirtuin family members. Extensively validiated for sirtuins 1-3. Unparalleled Flexibility – use of unlabeled substrates enables assay customization and the analysis of a variety of peptide substrates. High Throughput – 96-well plate format ideal for inhibitor/activator assays and the study of enzyme mechanisms. Assay design can be adapted to 384-well plates. Easy-to-use – simple protocol, homogeneous, no wash format. Reliable Chemistry – unlabeled peptide substrate avoids detection artifacts due compounds such as resveratrol. Rapid Results –easy two step assay with minimal hands on time allows you to go from sample to results in about 90 minutes. The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a single pre-labeled fluorescently tagged substrate, the SIRTainty Class III HDAC Assay employs untagged acetylated peptide substrate for the detection sirtuin activity. This approach not only enables unparalleled flexibility in the choice of sirtuin isoform and peptide substrate, but also eliminates the potential for artifacts due to the use of artificial substrates containing bulky fluorophores.
Alternate Names	SIRT HDAC assay Sirtuin assay Sirtuin HDAC assay SIRT assay
背景信息	Sirtuin, or Sir2 proteins, are a class of proteins that possess either mono-ADP-ribosyltransferase or deacylase activity, including deacetylase, desuccinylase, demalonylase, demyristoylase and depalmitoylase activity. Sirtuins have been implicated in influencing a wide range of cellular processes including aging, transcription, apoptosis, inflammation, stress resistance, as well as energy efficiency and alertness during low-calorie situations. Sirtuins can also control circadian clocks and mitochondrial biogenesis. The sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. The dependence of sirtuins on NAD links their enzymatic activity directly to the energy status of the cell via the cellular NAD:NADH ratio, the absolute levels of NAD, NADH or nicotinamide or a combination of these variables.

产品信息
部件	Developer Reagent Black 96 well plate and covers SIRTaintyTM Assay Buffer Acetylated Peptide Substrate β-NAD Nicotinamide (NAM) Recombinant Sirt1 Enzyme Recombinant Nicotinamidase Enzyme Suramin (Sirt Inhibitor)
演示	Single box containing all key reagents for evaluting the activity of class III histone deacetylases (sirtuins). Assay format is ideal for screening activators and inhibitors of all known sirtuin enzymes (sirts 1-7). Kit includes, sirtuin inhibitor, nicotinamide standard for quantitative analysis, and recombinant Sirt1 enzyme sufficient for proccessing 96 samples using 1.5 units of enzyme per assay.

产品信息

部件

Developer Reagent
Black 96 well plate and covers
SIRTaintyTM Assay Buffer
Acetylated Peptide Substrate
β-NAD
Nicotinamide (NAM)
Recombinant Sirt1 Enzyme
Recombinant Nicotinamidase Enzyme
Suramin (Sirt Inhibitor)

演示

Single box containing all key reagents for evaluting the activity of class III histone deacetylases (sirtuins). Assay format is ideal for screening activators and inhibitors of all known sirtuin enzymes (sirts 1-7). Kit includes, sirtuin inhibitor, nicotinamide standard for quantitative analysis, and recombinant Sirt1 enzyme sufficient for proccessing 96 samples using 1.5 units of enzyme per assay.

应用
应用	Class III HDAC assay used for the detection sirtuin activity levels. Assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes.
主要应用	Enzyme Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain the unopened kit at -80ºC. Plates and covers can be stored at room temperature (18-25º C). Unused components should be stored at -80ºC. When stored properly this kit is good for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles, aliquot if necessary.