merck millipore,默克密理博,AB1870,Anti-Osteopontin Antibody

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Merck Millipore 默克密理愽

Replacement Information

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ELISA, IHC	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	AB1870
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Osteopontin Antibody
Alternate Names	secreted phosphoprotein-1 (osteopontin, bone sialoprotein) Uropontin Bone sialoprotein 1 SPP1/CALPHA1 fusion Urinary stone protein bone sialoprotein I osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein secreted phosphoprotein 1 secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early<br />T-lymphocyte activation 1) early<br />T-lymphocyte activation 1) secreted phosphoprotein-1 (osteopontin
背景信息	Osteopontin is a single-chain polypeptide with a molecular weight of approximately 35 kDa. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.

参考

产品信息
格式	Serum
控制	POSITIVE CONTROL: Human urine (2-5 µg/mL normal concentration).
演示	Rabbit polyclonal antiserum in buffer containing no preservatives.

应用
应用	This Anti-Osteopontin Antibody is validated for use in ELISA, IH, IH(P), WB for the detection of Osteopontin.
主要应用	Western Blotting ELISA Immunohistochemistry
应用说明	Immunohistochemistry: A previous lot of this antibodu was used at a 1:1500 dilution on paraffin-embedded tissue sections. ELISA: A previous lot of this antibody was used at a 1:1000 dilution. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Synthetic peptide corresponding to amino acids 75-90 of human osteopontin (SwissProt Accession # P10451; PSKSNESHDHMDDM DD).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Human osteopontin. Does not cross react with osteonectin or human bone sialoprotein II.
品种反应性	RatHumanMouse
Species Reactivity Note	Human and Mouse. Expected to cross-react with rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_000573
基因符号	ETA-1 SPP1 BNSP MGC110940 Uropontin Nephropontin CIT:779436 osteopontin SPP-1 BSPI OPN
纯化方法	Unpurified
UniProt编号	P10451
UniProt汇总	FUNCTION: SwissProt: P10451 # Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity). SIZE: 314 amino acids; 35423 Da SUBUNIT: Ligand for integrin alpha-V/beta-3. SUBCELLULAR LOCATION: Secreted. PTM: Extensively phosphorylated on clustered serine residues. & N- and O-glycosylated. DISEASE:SwissProt: P10451 # This protein plays a principal role in urinary stone formation as the stone matrix. SIMILARITY: SwissProt: P10451 ## Belongs to the osteopontin family.

理化信息

尺寸

材料信息

毒性学信息

GHS安全信息

安全信息

产品使用声明
质量保证	Routinely evaluated by Western Blot on RAW 264.7 lysates. Western Blot Analysis: 1:500 dilution of this lot detected osteopontin on 10 μg of RAW 264.7 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	100 µL

运输信息

补充信息

规格

merck millipore,默克密理博,AB1870,Anti-Osteopontin Antibody

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