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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1476 Replaces 04-376 描述 Anti-MEKK1 Antibody Alternate Names - mitogen-activated protein kinase kinase kinase 1
- MEKK 1
- MEK kinase 1
- MAPK/ERK kinase kinase 1
- MAP/ERK kinase kinase 1
背景信息 MEKK1 (also known as Mitogen-activated protein kinase kinase kinase, MEK Kinase or MAP Kinase Kinase Kinase) is member of the serine/threonine protein kinase family and is a target in the JNK and ERK pathways. Phosphorylation of MEKK1 works with other kinases to activate cellular responses to mitogenic and metabolic stimuli. 产品信息 格式 Affinity Purified 控制 - PC3 cell lysate
演示 Purified rabbit polyclonal in borate buffered saline, pH 8.4, with no preservatives. 应用 应用 Detect MEKK1 using this Anti-MEKK1 Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to mouse MEKK1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes MEKK1. Antibody recognizes ~78 kDa and 180 kDa, corresponding to the apparent molecular masses of MEKK1 protein and cleaved CT fragment when assessed by Western blotting analysis. Other cross reactive bands are detected on immunoblot analysis. Based on predicted sequence homologies, no cross-reactivity with MEKK22 or MEKK3 is expected. 品种反应性 RatHumanMouse Species Reactivity Note Reacts with human. Expected to react with mouse and rat based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036075.2
基因符号 - OTTHUMP00000065818
- EC 2.7.11.25
- MAPKKK1
- MEKK
- MEKK1
纯化方法 Affinity Purfied UniProt编号 - Q13233
UniProt汇总 FUNCTION: Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by autophosphorylation on Thr-1400 and Thr-1412 following oligomerization.
SUBUNIT STRUCTURE: Binds both upstream activators and downstream substrates in multimolecular complexes through its N-terminus. Oligomerizes after binding MAP4K2 or TRAF2. Interacts with AXIN1.
PTM: Autophosphorylated By similarity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 RING-type zinc finger.
Contains 1 SWIM-type zinc finger.产品使用声明 质量保证 Evaluated by Western Blot in PC3 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MEKK1 on 10 µg of PC3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 12 months at -20°C from date of receipt. 包装信息 数量 50 µg
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