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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT178 描述 Anti-ADAMTS-4 Antibody Alternate Names - Aggrecanase-1
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- ADAM-TS 4
- ADAM-TS4
- ADAMTS-4
- ADMP-1
背景信息 The ADAMTS (A Disintegrin and Metalloprotease with Thrombospondin motifs) family of secreted proteases comprises at least 20 members in mammals, and several members in invertebrates. ADAMTS members resemble the membrane-bound ADAM family of proteases in the presence of a prodomain, a catalytic domain and a disintegrin-like domain. However, ADAMTS members lack a transmembrane domain, and instead contain varying numbers of thrombospondin type I modules. ADAMTS-4 was first purified by its ability to cleave aggrecan, a major cartilage proteoglycan that is degraded in patients with osteoarthritis and rheumatoid arthritis. ADAMTS-4 cleaves aggrecan between Glu373 and Ala374, a physiological cleavage site in arthritic cartilage. In addition, expression of ADAMTS-4 is elevated in chondrocytes treated with inflammatory cytokines interleukin-1, tumor necrosis factor and oncostatin M. Activation of the aggrecanase activity of ADAMTS-4 requires cleavage of the prodomain by a furin-like protease and the C-terminal domain by an as-yet-unidentified matrix metalloproteinase. Inclusion of protease inhibitors, including EDTA or other metalloprotease inhibitor, in the lysis buffer is recommended to inhibit proteolysis during lysate preparation. 产品信息 格式 Affinity Purified 演示 Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ADAMTS-4 Antibody is a highly specific rabbit polyclonal antibody, that targets ADAM TS4 & has been tested in western blotting. 主要应用 - Western Blotting
应用说明 Western Blotting Analysis: A 1:500 dilution from a representative lot detected ADAMTS-4 in 10 µg of human heart tissue lysate.
Western Blotting Analysis: A 1:250 dilution from a representative lot detected ADAMTS-4 in 50 ng of Recombinant Protein (AP182P).生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the Internal (Domain of Peptidase M12B) of human ADAMTS-4. 表位 Internal (Domain of Peptidase M12B) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005090
基因符号 - UNQ769/PRO1563
- ADAMTS4
- KIAA0688
纯化方法 Antigen Affinity purified UniProt编号 - O75173
产品使用声明 质量保证 Evaluated by Western Blotting in human chondrocytes cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ADAMTS-4 in 10 µg of human chondrocytes cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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